Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Identification of Gal4 activation domain-binding proteins in the 26S proteasome by periodate-triggered cross-linking

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Archer, C. T.
  • Burdine, L.
  • Kodadek, Thomas

publication date

  • December 2005

journal

  • Molecular Biosystems  Journal

abstract

  • A common occurrence in biology is that a regulatory peptide, protein, or small molecule regulates the activity of a large multi-protein complex through direct interactions with a protein(s) in that complex. To characterize the direct receptor of the regulatory molecule, one would ideally like to study the native system. We report here that periodate-triggered cross-linking of catechol-containing regulatory factors, followed by two-dimensional electrophoresis and Western blotting, is an effective method for the characterization of regulatory factor--protein interactions in the context of large multi-protein complexes. We demonstrate the utility of this methodology by identifying the Rpt6/Sug1 and Rpt4/Sug2 proteins as the direct targets of transcriptional activation domains in the 26S proteasome.

subject areas

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Binding Sites
  • Cross-Linking Reagents
  • DNA-Binding Proteins
  • Dihydroxyphenylalanine
  • Models, Molecular
  • Molecular Sequence Data
  • Peptides
  • Proteasome Endopeptidase Complex
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 1742-206X

Digital Object Identifier (DOI)

  • 10.1039/b510019d

PubMed ID

  • 16881005
scroll to property group menus

Additional Document Info

start page

  • 366

end page

  • 372

volume

  • 1

issue

  • 5-6

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support