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Monoclonal antibodies to a nuclear protein (PCNA/cyclin) associated with DNA replication

Academic Article
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Overview

authors

  • Ogata, K.
  • Kurki, P.
  • Celis, J. E.
  • Nakamura, R. M.
  • Tan, Eng

publication date

  • 1987

journal

  • Experimental Cell Research  Journal

abstract

  • Two hybridomas producing monoclonal antibodies to proliferating cell nuclear antigen. (PNCA)/cyclin were generated from spleen cells of BALB/c mice immunized with purified PCNA from rabbit thymus. The specificity of the monoclonal antibodies (19A2 and 19F4) was established by showing that they reacted in enzyme-linked immunosorbent assay (ELISA) with purified PCNA. Furthermore, they reacted in one-dimensional (ID) gel immunoblots with a 36 kD polypeptide which also reacted with human autoantibodies from lupus patients. Both monoclonals also recognized the nuclear polypeptide cyclin in two-dimensional (2D) gel immunoblots of HeLa cell proteins. Epitopes recognized by 19A2 and 19F4 were analysed by competitive inhibition test using a modified ELISA. The data suggested that the epitopes were closely related, but not identical. The data with human auto-antibodies were more difficult to interpret, although it suggested that some human anti-PCNA may share epitopes with 19A2 and 19F4, but in addition recognize different epitopes on the PCNA molecule.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • DNA Replication
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fluorescent Antibody Technique
  • HeLa Cells
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Molecular Weight
  • Nucleoproteins
  • Proliferating Cell Nuclear Antigen
  • Rabbits
  • Thymus Gland
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Identity

International Standard Serial Number (ISSN)

  • 0014-4827

Digital Object Identifier (DOI)

  • 10.1016/0014-4827(87)90020-6

PubMed ID

  • 2879737
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Additional Document Info

start page

  • 475

end page

  • 486

volume

  • 168

issue

  • 2

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