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Recognition of rna polymerase ii and transcription bubbles by xpg, csb, and tfiih: Insights for transcription-coupled repair and cockayne syndrome

Academic Article
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Overview

authors

  • Sarker, A. H.
  • Tsutakawa, S. E.
  • Kostek, S.
  • Ng, C.
  • Shin, D. S.
  • Peris, M.
  • Campeau, E.
  • Tainer, John
  • Nogales, E.
  • Cooper, P. K.

publication date

  • October 2005

journal

  • Molecular Cell  Journal

abstract

  • Loss of a nonenzymatic function of XPG results in defective transcription-coupled repair (TCR), Cockayne syndrome (CS), and early death, but the molecular basis for these phenotypes is unknown. Mutation of CSB, CSA, or the TFIIH helicases XPB and XPD can also cause defective TCR and CS. We show that XPG interacts with elongating RNA polymerase II (RNAPII) in the cell and binds stalled RNAPII ternary complexes in vitro both independently and cooperatively with CSB. XPG binds transcription-sized DNA bubbles through two domains not required for incision and functionally interacts with CSB on these bubbles to stimulate its ATPase activity. Bound RNAPII blocks bubble incision by XPG, but an ATP hydrolysis-dependent process involving TFIIH creates access to the junction, allowing incision. Together, these results implicate coordinated recognition of stalled transcription by XPG and CSB in TCR initiation and suggest that TFIIH-dependent remodeling of stalled RNAPII without release may be sufficient to allow repair.

subject areas

  • Adenosine Triphosphatases
  • Cockayne Syndrome
  • Crystallography, X-Ray
  • DNA Repair
  • DNA-Binding Proteins
  • Endonucleases
  • HeLa Cells
  • Humans
  • Models, Molecular
  • Mutation
  • Nuclear Proteins
  • RNA Polymerase II
  • Time Factors
  • Transcription Factor TFIIH
  • Transcription Factors
  • Transcription, Genetic
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Identity

International Standard Serial Number (ISSN)

  • 1097-2765

Digital Object Identifier (DOI)

  • 10.1016/j.molcel.2005.09.022

PubMed ID

  • 16246722
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Additional Document Info

start page

  • 187

end page

  • 198

volume

  • 20

issue

  • 2

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