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Purified recombinant 60kd ss-a/ro protein - an antigenic source for elisa screening of patients with rheumatic diseases

Academic Article
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Overview

authors

  • Benchetrit, E.
  • Pollard, Kenneth Michael

publication date

  • August 1990

journal

  • Journal of Clinical & Laboratory Immunology  Journal

abstract

  • A 60 kD SS-A/Ro recombinant protein was expressed in E. coli cells and purified by the centrifugation procedure. Since the substrate contained a substantial amount of bacterial proteins it was not sufficient for ELISA. Therefore the material obtained by centrifugation was resolved on SDS-gel and the 60 kD region was excised and electro-eluted. Two hundred and nine sera from normal donors and patients with rheumatic diseases were analyzed by ELISA using the purified recombinant 60 kD SS-A/Ro protein. A comparison with Ouchterlony and Western blot revealed that ELISA proved to be a sensitive and specific method, which could discriminate between anti 60 kD antibodies and other autoantibodies. The present study showed that recombinant 60 kD may provide a purified source of antigen for ELISA while this method may serve as a useful tool for screening and evaluation of anti 60 kD antibodies and their relevance to clinical manifestations.

subject areas

  • Autoantigens
  • Centrifugation
  • DNA
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • RNA, Small Cytoplasmic
  • Recombinant Proteins
  • Rheumatic Diseases
  • Ribonucleoproteins
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Research

keywords

  • 60KD SS-A/RO ANTIGEN
  • ELISA
  • RECOMBINANT PROTEIN
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Identity

International Standard Serial Number (ISSN)

  • 0141-2760

PubMed ID

  • 1966938
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Additional Document Info

start page

  • 195

end page

  • 200

volume

  • 32

issue

  • 4

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