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NMR structure of the integral membrane protein OmpX

Academic Article
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Overview

authors

  • Fernandez, C.
  • Hilty, C.
  • Wider, G.
  • Guntert, P.
  • Wuthrich, Kurt

publication date

  • March 2004

journal

  • Journal of Molecular Biology  Journal

abstract

  • The structure of the integral membrane protein OmpX from Escherichia coli reconstituted in 60 kDa DHPC micelles (OmpX/DHPC) was calculated from 526 NOE upper limit distance constraints. The structure determination was based on complete sequence-specific assignments for the amide protons and the Val, Leu, and Ile(delta1) methyl groups in OmpX, which were selectively protonated on a perdeuterated background. The solution structure of OmpX in the DHPC micelles consists of a well-defined, eight-stranded antiparallel beta-barrel, with successive pairs of beta-strands connected by mobile loops. Several long-range NOEs observed outside of the transmembrane barrel characterize an extension of a four-stranded beta-sheet beyond the height of the barrel. This protruding beta-sheet is believed to be involved in intermolecular interactions responsible for the biological functions of OmpX. The present approach for de novo structure determination should be quite widely applicable to membrane proteins reconstituted in mixed micelles with overall molecular masses up to about 100 kDa, and may also provide a platform for additional functional studies.

subject areas

  • Amino Acid Sequence
  • Bacterial Outer Membrane Proteins
  • Escherichia coli Proteins
  • Hydrolases
  • Micelles
  • Models, Molecular
  • Nuclear Magnetic Resonance, Biomolecular
  • Phospholipid Ethers
  • Protein Conformation
  • Protein Structure, Secondary
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Research

keywords

  • OmpX
  • TROSY
  • membrane proteins
  • micelles
  • protein structure
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Identity

International Standard Serial Number (ISSN)

  • 0022-2836

Digital Object Identifier (DOI)

  • 10.1016/j.jmb.2003.09.014

PubMed ID

  • 15037080
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Additional Document Info

start page

  • 1211

end page

  • 1221

volume

  • 336

issue

  • 5

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