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D-AKAP2, a novel protein kinase A anchoring protein with a putative RGS domain

Academic Article
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Overview

authors

  • Huang, L. J. S.
  • Durick, K.
  • Weiner, J. A.
  • Chun, Jerold
  • Taylor, S. S.

publication date

  • October 1997

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Subcellular localization directed by specific A kinase anchoring proteins (AKAPs) is a mechanism for compartmentalization of cAMP-dependent protein kinase (PKA). Using a two-hybrid screen, a novel AKAP was isolated. Because it interacts with both the type I and type II regulatory subunits, it was defined as a dual specific AKAP or D-AKAP1. Here we report the cloning and characterization of another novel cDNA isolated from that screen. This new member of the D-AKAP family, D-AKAP2, also binds both types of regulatory subunits. A message of 5 kb pairs was detected for D-AKAP2 in all embryonic stages and in all adult tissues tested. In brain, skeletal muscle, kidney, and testis, a 10-kb mRNA was identified. In testis, several small mRNAs were observed. Therefore, D-AKAP2 represents a novel family of proteins. cDNA cloning from a mouse testis library identified the full length D-AKAP2. It is composed of 372 amino acids which includes the R binding fragment, residues 333-372, at its C-terminus. Based on coprecipitation assays, the R binding domain interacts with the N-terminal dimerization domain of RIalpha and RIIalpha. A putative RGS domain was identified near the N-terminal region of D-AKAP2. The presence of this domain raises the intriguing possibility that D-AKAP2 may interact with a Galpha protein thus providing a link between the signaling machinery at the plasma membrane and the downstream kinase.

subject areas

  • A Kinase Anchor Proteins
  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Carrier Proteins
  • Cloning, Molecular
  • Conserved Sequence
  • Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit
  • Cyclic AMP-Dependent Protein Kinase RIalpha Subunit
  • Cyclic AMP-Dependent Protein Kinase Type II
  • Cyclic AMP-Dependent Protein Kinases
  • DNA, Complementary
  • Embryo, Mammalian
  • GTPase-Activating Proteins
  • Gene Library
  • Macromolecular Substances
  • Male
  • Mice
  • Molecular Sequence Data
  • Organ Specificity
  • Proteins
  • RNA, Messenger
  • Recombinant Proteins
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Substrate Specificity
  • Testis
  • Transcription, Genetic
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.94.21.11184

PubMed ID

  • 9326583
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Additional Document Info

start page

  • 11184

end page

  • 11189

volume

  • 94

issue

  • 21

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