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Development and maintenance of a b220(-) memory b cell compartment

Academic Article
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Overview

authors

  • Driver, D. J.
  • McHeyzer-Williams, L. J.
  • Cool, M.
  • Stetson, D. B.
  • McHeyzer-Williams, Michael G.

publication date

  • August 2001

journal

  • Journal of Immunology  Journal

abstract

  • We have recently demonstrated that a novel somatically mutated B220(-) memory B cell subset rapidly dominates the secondary immune response to (4-hydroxy-3-nitrophenyl) acetyl (NP). Upon adoptive transfer with Ag, B220(+)NP(+) memory B cells produce large numbers of B220(-)NP(+) B cells that can rapidly differentiate into plasma cells. Therefore, it is not clear whether the novel B220(-) memory compartment is a consequence of secondary Ag challenge or whether it develops as a stable memory subset after initial Ag challenge. In this study, we demonstrate the gradual emergence of B220(-)NP(+) B cells in the spleen to maximal numbers 3 wk after initial Ag exposure. Like their B220(+) counterparts, the B220(-) B cells initially appear unmutated at days 5-7; however, the majority rapidly accumulate affinity increasing mutations by days 9-14 of the primary immune response. More extensive cell surface phenotype (GL7(-)BLA-1(-)CD24(-)CD43(+)) argues strongly against germinal center localization and direct analysis in situ places a cohort of B220(-)CD11b(+)NP(+) B cells in the red pulp of the spleen and not in the MZs. These data provide direct evidence for the development of B220(-) memory B cells as a unique cellular consequence of primary Ag exposure. The cellular dynamics and molecular attributes of these unique memory B cells suggest they are distinct cellular products of the germinal center reaction in the primary response and are maintained long-term in the spleen and bone marrow.

subject areas

  • Amino Acid Sequence
  • Animals
  • Antigens, CD45
  • B-Lymphocyte Subsets
  • Base Sequence
  • Bone Marrow Cells
  • Cell Differentiation
  • Cell Survival
  • Epitopes, B-Lymphocyte
  • Female
  • Germinal Center
  • Haptens
  • Immunoglobulin E
  • Immunoglobulin Heavy Chains
  • Immunoglobulin lambda-Chains
  • Immunologic Memory
  • Immunophenotyping
  • Macrophage-1 Antigen
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutation
  • Nitrophenols
  • Phenylacetates
  • Spleen
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 11466358
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Additional Document Info

start page

  • 1393

end page

  • 1405

volume

  • 167

issue

  • 3

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