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A large array of human monoclonal-antibodies to type-1 human-immunodeficiency-virus from combinatorial libraries of asymptomatic seropositive individuals

Academic Article
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Overview

authors

  • Burton, Dennis
  • Barbas III, Carlos
  • Persson, M. A. A.
  • Koenig, S.
  • Chanock, R. M.
  • Lerner, Richard

publication date

  • November 1991

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • A panel of human monoclonal antibody Fab fragments has been generated against the surface glycoprotein gp120 of type 1 human immunodeficiency virus (HIV) by antigen selection from a random combinatorial library expressed on the surface of filamentous phage. The library was prepared from 5 ml of bone marrow from an asymptomatic individual who has been HIV-positive for 6 years. The antibodies have high affinity for antigen (mostly with affinity constants of greater than 10(8) M-1) and notable sequence diversity. Given appropriate donor selection, the methods described should allow the generation of antibodies for the evaluation of passive immunization as a therapy for AIDS.

subject areas

  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Binding, Competitive
  • Cloning, Molecular
  • Colony-Forming Units Assay
  • Enzyme-Linked Immunosorbent Assay
  • Gene Library
  • HIV Antibodies
  • HIV Envelope Protein gp120
  • HIV Seropositivity
  • HIV-1
  • Immunoglobulin Fab Fragments
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Lymphocytes
  • Molecular Sequence Data
  • RNA
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Research

keywords

  • AIDS
  • ANTIBODY REPERTOIRES
  • FILAMENTOUS PHAGE
  • PASSIVE IMMUNIZATION
  • PHAGE SURFACE EXPRESSION
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.88.22.10134

PubMed ID

  • 1719545
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Additional Document Info

start page

  • 10134

end page

  • 10137

volume

  • 88

issue

  • 22

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