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Crystallization and preliminary crystallographic data for rab guanine-nucleotide dissociation inhibitor (rabgdi) from bovine brain

Academic Article
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Overview

authors

  • Schalk, I. J.
  • Stura, E. A.
  • Matteson, J.
  • Wilson, Ian
  • Balch, William E.

publication date

  • December 1994

journal

  • Journal of Molecular Biology  Journal

abstract

  • X-ray quality crystals of Rab guanine nucleotide dissociation inhibitor (RabGDI) from bovine brain expressed in Escherichia coli have been obtained from 1.73 M ammonium sulfate. The crystals are prismatic long rods and belong to the monoclinic space group P21 with approximate cell dimensions a = 91.9 A, b = 43.5 A, c = 63.2 A, beta = 104.5 degrees and one molecule per asymmetric unit. The crystals are stable in the X-ray beam and diffract to at least 2.3 A. Reverse screening, streak seeding and macroseeding methods were used to obtain and improve the crystals.

subject areas

  • Animals
  • Brain Chemistry
  • Cattle
  • Crystallography, X-Ray
  • GTP-Binding Proteins
  • Guanine Nucleotide Dissociation Inhibitors
  • rab3 GTP-Binding Proteins
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Research

keywords

  • CRYSTALLIZATION SCREENING
  • INTRACELLULAR VESICULAR TRAFFIC
  • PHOSPHOLIPID
  • RAB3
  • RABGDI
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Identity

International Standard Serial Number (ISSN)

  • 0022-2836

Digital Object Identifier (DOI)

  • 10.1006/jmbi.1994.1744

PubMed ID

  • 7990134
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Additional Document Info

start page

  • 469

end page

  • 473

volume

  • 244

issue

  • 4

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