Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Mixture analysis using a quadrupole mass filter quadrupole ion trap mass spectrometer

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Jonscher, K. R.
  • Yates III, John

publication date

  • February 1996

journal

  • Analytical Chemistry  Journal

abstract

  • A hybrid tandem mass spectrometer is constructed by interfacing a quadrupole mass filter (Q) to a quadrupole ion trap mass spectrometer (QITMS) and is evaluated for the analysis of mixtures. The mass filter is set to selectively inject ions of a particular m/z or, in scanning mode, to sequentially inject ions into the QITMS for subsequent manipulation and detection. Performance of the instrument is demonstrated using a mixture of ions created by electron impact ionization of perfluorotributylamine (FC-43) and peptide ions generated by pulsed Cs+ bombardment. Resulting data are compared to those obtained by utilizing only the ion trap. Molecular weight, fragmentation, and high-resolution analyses for the sequentially injected mass-filtered peptides show improved performance over similar measurements employing only the ion trap mass spectrometer. Performance is optimized when ions are not rf-isolated in the QITMS. Using the hybrid, a resolution of 33,200 is achieved for angiotensin I. Dramatic reduction of space charge-induced signal suppression is demonstrated for LSIMS of Glu-fibrinopeptide B. 'On-the-fly' collision-induced dissociation is performed for m/z 502 from FC-43, where fragmentation is induced by increasing the ion injection energy. Collision-induced dissociation efficiencies for fragmentation of angiotensin I by resonance excitation are investigated as a function of cooling time for different modes of operation of the hybrid. A current limitation of the instrument is the time required to port the data for acquisition.

subject areas

  • Amino Acid Sequence
  • Angiotensin I
  • Humans
  • Mass Spectrometry
  • Molecular Sequence Data
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac950978s

PubMed ID

  • 8999740
scroll to property group menus

Additional Document Info

start page

  • 659

end page

  • 667

volume

  • 68

issue

  • 4

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support