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Recombination signatures distinguish embryonic stem cells derived by parthenogenesis and somatic cell nuclear transfer

Academic Article
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Overview

authors

  • Kim, K.
  • Ng, K.
  • Rugg-Gunn, P. J.
  • Shieh, J. H.
  • Kirak, Oktay
  • Jaenisch, R.
  • Wakayama, T.
  • Moore, M. A.
  • Pedersen, R. A.
  • Daley, G. Q.

publication date

  • September 2007

journal

  • Cell Stem Cell  Journal

abstract

  • Parthenogenesis and somatic cell nuclear transfer (SCNT) are two methods for deriving embryonic stem (ES) cells that are genetically matched to the oocyte donor or somatic cell donor, respectively. Using genome-wide single nucleotide polymorphism (SNP) analysis, we demonstrate distinct signatures of genetic recombination that distinguish parthenogenetic ES cells from those generated by SCNT. We applied SNP analysis to the human ES cell line SCNT-hES-1, previously claimed to have been derived by SCNT, and present evidence that it represents a human parthenogenetic ES cell line. Genome-wide SNP analysis represents a means to validate the genetic provenance of an ES cell line.

subject areas

  • Animals
  • Cell Line
  • Cytogenetic Analysis
  • DNA Methylation
  • Embryonic Stem Cells
  • Genome, Human
  • Heterozygote
  • Homozygote
  • Humans
  • Mice
  • Nuclear Transfer Techniques
  • Parthenogenesis
  • Polymorphism, Single Nucleotide
  • Recombination, Genetic
  • Sequence Analysis, DNA
  • Specimen Handling
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Identity

International Standard Serial Number (ISSN)

  • 1934-5909

Digital Object Identifier (DOI)

  • 10.1016/j.stem.2007.07.001

PubMed ID

  • 18371368
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Additional Document Info

start page

  • 346

end page

  • 352

volume

  • 1

issue

  • 3

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