Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Substrate sequence effects on hammerhead RNA catalytic efficiency

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Fedor, Martha
  • Uhlenbeck, O. C.

publication date

  • March 1990

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • The "hammerhead" RNA self-cleaving domain can be assembled from two RNA molecules: a large (approximately 34 nucleotide) ribozyme RNA containing most of the catalytically essential nucleotides and a small (approximately 13 nucleotide) substrate RNA containing the cleavage site. Four such hammerheads that contained identical catalytic core sequences but differed in the base composition of the helices that are involved in substrate binding had been reported to vary in cleavage rates by more than 70-fold under similar reaction conditions. Steady-state kinetic analyses reveal that kcat values are nearly the same for these hammerheads but Km values vary nearly 60-fold. The substrates for reactions having high Km values form aggregates that are virtually nonreactive. These observations demonstrate that the secondary structure of substrate RNA can be a major determinant of hammerhead catalytic efficiency.

subject areas

  • Base Composition
  • Base Sequence
  • Electrophoresis, Polyacrylamide Gel
  • Kinetics
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • RNA
  • RNA Splicing
  • RNA, Catalytic
  • RNA, Ribosomal
  • Transcription, Genetic
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.87.5.1668

PubMed ID

  • 1689847
scroll to property group menus

Additional Document Info

start page

  • 1668

end page

  • 1672

volume

  • 87

issue

  • 5

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support