Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Competition between Xenopus satellite-I sequences and Pol-III genes for stable transcription complex-formation

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Andrews, D. L.
  • Millstein, L.
  • Hamkalo, B. A.
  • Gottesfeld, Joel

publication date

  • 1984

journal

  • Nucleic Acids Research  Journal

abstract

  • We have constructed hybrid plasmids bearing both Xenopus 5S RNA genes and satellite I sequences in order to test the effect of satellite DNA on 5S gene transcription. Satellite sequences inactivate 5S transcription in both HeLa S100 and Xenopus oocyte microinjection transcription assays. Inactivation of 5S transcription by satellite DNA is observed both in cis and in trans. Transcription of a tRNA gene is also precluded by satellite I DNA. The Xenopus satellite I repeat contains an RNA polymerase III transcription unit which is highly active in both assay systems. This promoter element is 10- to 25-fold more efficient than the 5S gene in transcription competition assays. This quantitative difference in affinity for transcription components may explain the inactivation of 5S transcription by satellite sequences. The satellite I promoter forms stable transcription complexes in vitro which do not dissociate for at least 30 rounds of transcription. Although stable complex formation on the satellite promoter is largely temperature independent over the range of 0-20 degrees, complex formation on both 5S and tRNA genes exhibits a steep temperature dependence characteristic of DNA helix unwinding. The DNA sequence requirements for stable complex formation on 5S genes have been determined using 5' deletion mutants.

subject areas

  • Animals
  • Cloning, Molecular
  • DNA Polymerase III
  • DNA Restriction Enzymes
  • DNA, Recombinant
  • DNA, Satellite
  • DNA-Directed DNA Polymerase
  • Female
  • Genes
  • HeLa Cells
  • Humans
  • Nucleic Acid Hybridization
  • Oocytes
  • Plasmids
  • Transcription, Genetic
  • Xenopus
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0305-1048

Digital Object Identifier (DOI)

  • 10.1093/nar/12.20.7753

PubMed ID

  • 6093052
scroll to property group menus

Additional Document Info

start page

  • 7753

end page

  • 7769

volume

  • 12

issue

  • 20

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support