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The long third complementarity-determining region of the heavy chain is important in the activity of the broadly neutralizing anti-human immunodeficiency virus type 1 antibody 2f5

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Overview

related to degree

  • Komori-Occhicone, Heather Kiyomi, Ph.D. in Biology, Scripps Research 2002 - 2008

authors

  • Zwick, Michael
  • Komori-Occhicone, Heather Kiyomi
  • Stanfield, Robyn
  • Church, S.
  • Wang, M.
  • Parren, P. W. H. I.
  • Kunert, R.
  • Katinger, H.
  • Wilson, Ian
  • Burton, Dennis

publication date

  • March 2004

journal

  • Journal of Virology  Journal

abstract

  • The human monoclonal antibody 2F5 neutralizes primary human immunodeficiency virus type 1 (HIV-1) with rare breadth and potency. A crystal structure of a complex of 2F5 and a peptide corresponding to its core epitope on gp41, ELDKWAS, revealed that the peptide interacts with residues at the base of the unusually long (22-residue) third complementarity-determining region of the heavy chain (CDR H3) but not the apex. Here, we perform alanine-scanning mutagenesis across CDR H3 and make additional substitutions of selected residues to map the paratope of Fab 2F5. Substitution of residues from the base of the H3 loop or from CDRs H1, H2, and L3, which are proximal to the peptide, significantly diminished the affinity of Fab 2F5 for gp41 and a short peptide containing the 2F5 core motif. However, nonconservative substitutions to a phenylalanine residue at the apex of the H3 loop also markedly decreased 2F5 binding to both gp41 and the peptide, suggesting that recognition of the core epitope is crucially dependent on features at the apex of the H3 loop. Furthermore, substitution at the apex of the H3 loop had an even more pronounced effect on the neutralizing activity of 2F5 against three sensitive HIV-1. These observations present a challenge to vaccine strategies based on peptide mimics of the linear epitope.

subject areas

  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Complementarity Determining Regions
  • Epitopes
  • HIV Antibodies
  • HIV Envelope Protein gp41
  • Humans
  • Models, Molecular
  • Molecular Sequence Data
  • Mutagenesis
  • Neutralization Tests
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Identity

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.78.6.3155-3161.2004

PubMed ID

  • 14990736
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Additional Document Info

start page

  • 3155

end page

  • 3161

volume

  • 78

issue

  • 6

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