Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Genetic modulation of T cell receptor gene segment usage during somatic recombination

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Livak, F.
  • Burtrum, D. B.
  • Rowen, L.
  • Schatz, D. G.
  • Petrie, Howard

publication date

  • October 2000

journal

  • Journal of Experimental Medicine  Journal

abstract

  • Lymphocyte antigen receptors are not encoded by germline genes, but rather are produced by combinatorial joining between clusters of gene segments in somatic cells. Within a given cluster, gene segment usage during recombination is thought to be largely random, with biased representation in mature T lymphocytes resulting from protein-mediated selection of a subset of the total repertoire. Here we show that T cell receptor D beta and J beta gene segment usage is not random, but is patterned at the time of recombination. The hierarchy of gene segment usage is independent of gene segment proximity, but rather is influenced by the ability of the flanking recombination signal sequences (RSS) to bind the recombinase and/or to form a paired synaptic complex. Importantly, the relative frequency of gene segment usage established during recombination is very similar to that found after protein-mediated selection, suggesting that in addition to targeting recombinase activity, the RSS may have evolved to bias the naive repertoire in favor of useful gene products.

subject areas

  • Animals
  • Base Sequence
  • Consensus Sequence
  • DNA Primers
  • DNA, Ribosomal
  • Genes, T-Cell Receptor
  • Genes, T-Cell Receptor beta
  • Kidney
  • Mice
  • Mice, Inbred C57BL
  • Polymerase Chain Reaction
  • Recombination, Genetic
  • T-Lymphocytes
scroll to property group menus

Research

keywords

  • T cell receptor beta locus
  • VDJ recombination
  • recombination signal sequence
  • repertoire selection
  • thymus
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.192.8.1191

PubMed ID

  • 11034609
scroll to property group menus

Additional Document Info

start page

  • 1191

end page

  • 1196

volume

  • 192

issue

  • 8

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support