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Structural and functional identification of major histocompatibility complex class i-restricted self-peptides as naturally occurring molecular mimics of viral antigens - possible role in cd8(+) t cell-mediated, virus-induced autoimmune disease

Academic Article
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Overview

authors

  • Hudrisier, D.
  • Riond, J.
  • Burlet-Schiltz, O.
  • von Herrath, M. G.
  • Lewicki, H.
  • Monsarrat, B.
  • Oldstone, Michael
  • Gairin, J. E.

publication date

  • June 2001

journal

  • Journal of Biological Chemistry  Journal

abstract

  • Structural similarity (molecular mimicry) between viral epitopes and self-peptides can lead to the induction of autoaggressive CD4(+) as well as CD8(+) T cell responses. Based on the flexibility of T cell receptor/antigen/major histocompatibility complex recognition, it has been proposed that a self-peptide could replace a viral epitope for T cell recognition and therefore participate in pathophysiological processes in which T cells are involved. To address this issue, we used, as a molecular model of viral antigen, the H-2D(b)-restricted immunodominant epitope nucleoprotein (NP)-(396-404) (FQPQNGQFI) of lymphocytic choriomeningitis virus (LCMV). We identified peptide sequences from murine self-proteins that share structural and functional homology with LCMV NP-(396-404) and that bound to H-2D(b) with high affinity. One of these self-peptides, derived from tumor necrosis factor receptor I (FGPSNWHFM, amino acids 302-310), maintained LCMV-specific CD8(+) T cells in an active state as observed both in vitro in cytotoxic assays and in vivo in a model of virus-induced autoimmune diabetes, the rat insulin promoter-LCMV NP transgenic mouse. The natural occurrence and molecular concentration at the surface of H-2(b) spleen cells of tumor necrosis factor receptor I-(302-310) were determined by on-line micro-high pressure liquid chromatography/mass spectrometry and supported its biological relevance.

subject areas

  • Adoptive Transfer
  • Amino Acid Sequence
  • Animals
  • Antigens
  • Antigens, CD4
  • Autoimmune Diseases
  • CD8-Positive T-Lymphocytes
  • Cell Division
  • Cell Line
  • Chromatography, High Pressure Liquid
  • Dose-Response Relationship, Drug
  • Epitopes
  • Humans
  • Inhibitory Concentration 50
  • Insulin
  • Interferon-gamma
  • Major Histocompatibility Complex
  • Mass Spectrometry
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Models, Molecular
  • Molecular Sequence Data
  • Mutation
  • Peptides
  • Promoter Regions, Genetic
  • Protein Binding
  • Rats
  • Spleen
  • Structure-Activity Relationship
  • Time Factors
  • Transplantation
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.M008864200

PubMed ID

  • 11278441
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Additional Document Info

start page

  • 19396

end page

  • 19403

volume

  • 276

issue

  • 22

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