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A cell free system to study reassembly of the nuclear-envelope at the end of mitosis

Academic Article
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Overview

authors

  • Burke, B.
  • Gerace, Larry

publication date

  • February 1986

journal

  • Cell  Journal

abstract

  • We described a cell free system involving total homogenates of metaphase CHO cells, which yields telophase-like assembly of nuclear envelopes around mitotic chromosomes. During formation of the nuclear envelope in vitro, the three major lamina polypeptides (lamins A, B, and C) assemble around chromosomes and become dephosphorylated, similar to their behavior in vivo during telophase. Nuclear lamina and envelope assembly apparently do not require free ATP and are strongly inhibited by gamma-S-ATP, supporting the notion that these processes are regulated by protein dephosphorylation. Immunological depletion of disassembled lamins from the initial assembly system results in strong inhibition of subsequent nuclear envelope assembly, directly demonstrating that the lamins are involved in this process.

subject areas

  • Adenosine Triphosphate
  • Animals
  • Cell-Free System
  • Chromosomes
  • Cricetinae
  • Lamins
  • Metaphase
  • Mitosis
  • Morphogenesis
  • Nuclear Envelope
  • Nucleoproteins
  • Phosphorylation
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Identity

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(86)90273-4

PubMed ID

  • 3948244
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Additional Document Info

start page

  • 639

end page

  • 652

volume

  • 44

issue

  • 4

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