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Retrovirus-specific packaging of aminoacyl-tRNA synthetases with cognate primer tRNAs

Academic Article
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Overview

authors

  • Cen, S.
  • Javanbakht, H.
  • Kim, S.
  • Shiba, K.
  • Craven, R.
  • Rein, A.
  • Ewalt, K.
  • Schimmel, Paul
  • Musier-Forsyth, K.
  • Kleiman, L.

publication date

  • 2002

journal

  • Journal of Virology  Journal

abstract

  • The tRNAs used to prime reverse transcription in human immunodeficiency virus type 1 (HIV-1), Rous sarcoma virus (RSV), and Moloney murine leukemia virus (Mo-MuLV) are, tRNA(Trp), and tRNA(Pro), respectively. Using antibodies to the three cognate human aminoacyl-tRNA synthetases, we found that only lysyl-tRNA synthetase (LysRS) is present in HIV-1, only tryptophanyl-tRNA synthetase (TrpRS) is present in RSV, and neither these two synthetases nor prolyl-tRNA synthetase (ProRS) is present in Mo-MuLV. LysRS and TrpRS are present in HIV-1 and RSV at approximately 25 and 12 molecules/virion, respectively. These results support the hypothesis that, in HIV-1 and RSV, the cognate aminoacyl-tRNA synthetase may be used as the signal for targeting the selective packaging of primer tRNAs into retroviruses. The absence of ProRS in Mo-MuLV is consistent with reports that selective packaging of tRNA(Pro) in this virus is less important for achieving optimum annealing of the primer to Mo-MuLV genomic RNA.

subject areas

  • 3T3 Cells
  • Amino Acyl-tRNA Synthetases
  • Animals
  • Avian Sarcoma Viruses
  • HIV-1
  • Humans
  • Mice
  • Molecular Weight
  • Moloney murine leukemia virus
  • RNA, Transfer
  • RNA, Viral
  • Rabbits
  • Virus Assembly
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Identity

PubMed Central ID

  • PMC136713

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.76.24.13111-13115.2002

PubMed ID

  • 12438642
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Additional Document Info

start page

  • 13111

end page

  • 13115

volume

  • 76

issue

  • 24

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