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Functional characterization of the S-cerevisiae genome by gene deletion and parallel analysis

Academic Article
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Overview

authors

  • Winzeler, Elizabeth
  • Shoemaker, D. D.
  • Astromoff, A.
  • Liang, H.
  • Anderson, K.
  • Andre, B.
  • Bangham, R.
  • Benito, R.
  • Boeke, J. D.
  • Bussey, H.
  • Chu, A. M.
  • Connelly, C.
  • Davis, K.
  • Dietrich, F.
  • Dow, S. W.
  • El Bakkoury, M.
  • Foury, F.
  • Friend, S. H.
  • Gentalen, E.
  • Giaever, G.
  • Hegemann, J. H.
  • Jones, T.
  • Laub, M.
  • Liao, H.
  • Liebundguth, N.
  • Lockhart, D. J.
  • Lucau-Danila, A.
  • Lussier, M.
  • M'Rabet, N.
  • Menard, P.
  • Mittmann, M.
  • Pai, C.
  • Rebischung, C.
  • Revuelta, J. L.
  • Riles, L.
  • Roberts, C. J.
  • Ross-MacDonald, P.
  • Scherens, B.
  • Snyder, M.
  • Sookhai-Mahadeo, S.
  • Storms, R. K.
  • Veronneau, S.
  • Voet, M.
  • Volckaert, G.
  • Ward, T. R.
  • Wysocki, R.
  • Yen, G. S.
  • Yu, K. X.
  • Zimmermann, K.
  • Philippsen, P.
  • Johnston, M.
  • Davis, R. W.

publication date

  • August 1999

journal

  • Science  Journal

abstract

  • The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.

subject areas

  • Culture Media
  • Gene Deletion
  • Gene Expression Regulation, Fungal
  • Gene Targeting
  • Genes, Essential
  • Genes, Fungal
  • Genome, Fungal
  • Open Reading Frames
  • Phenotype
  • Polymerase Chain Reaction
  • Recombination, Genetic
  • Saccharomyces cerevisiae
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Identity

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.285.5429.901

PubMed ID

  • 10436161
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Additional Document Info

start page

  • 901

end page

  • 906

volume

  • 285

issue

  • 5429

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