Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Isolation of timeless by per protein-interaction - defective interaction between timeless protein and long-period mutant per(l)

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Gekakis, Nicholas
  • Saez, L.
  • Delahayebrown, A. M.
  • Myers, M. P.
  • Sehgal, A.
  • Young, M. W.
  • Weitz, C. J.

publication date

  • November 1995

journal

  • Science  Journal

abstract

  • The period (per) gene likely encodes a component of the Drosophila circadian clock. Circadian oscillations in the abundance of per messenger RNA and per protein (PER) are thought to arise from negative feedback control of per gene transcription by PER. A recently identified second clock locus, timeless (tim), apparently regulates entry of PER into the nucleus. Reported here are the cloning of complementary DNAs derived from the tim gene in a two-hybrid screen for PER-interacting proteins and the demonstration of a physical interaction between the tim protein (TIM) and PER in vitro. A restricted segment of TIM binds directly to a part of the PER dimerization domain PAS. PERL, a mutation that causes a temperature-sensitive lengthening of circadian period and a temperature-sensitive delay in PER nuclear entry, exhibits a temperature-sensitive defect in binding to TIM. These results suggest that the interaction between TIM and PER determines the timing of PER nuclear entry and therefore the duration of part of the circadian cycle.

subject areas

  • Animals
  • Biological Clocks
  • Cell Nucleus
  • Circadian Rhythm
  • Cloning, Molecular
  • Cytoplasm
  • DNA, Complementary
  • Drosophila Proteins
  • Drosophila melanogaster
  • Feedback
  • Gene Expression Regulation
  • Genes, Insect
  • Mutation
  • Nuclear Proteins
  • Period Circadian Proteins
  • Proteins
  • Recombinant Fusion Proteins
  • Temperature
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.270.5237.811

PubMed ID

  • 7481773
scroll to property group menus

Additional Document Info

start page

  • 811

end page

  • 815

volume

  • 270

issue

  • 5237

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support