Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

L-type Ca2+ channels contribute to current-evoked spike firing and associated Ca2+ signals in cerebellar Purkinje neurons

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Gruol, Donna
  • Netzeband, J. G.
  • Schneeloch, J.
  • Gullette, C. E.

publication date

  • June 2006

journal

  • Cerebellum  Journal

abstract

  • The physiological properties of Purkinje neurons play a central role in their ability to regulate information transfer through the cerebellum. A number of ion channels contribute to Purkinje neuron physiology including an abundance of P-type Ca2+ channels, particularly in the dendritic region. Purkinje neurons also express L-type Ca2+ channels both during development and in the mature state. However, a role for L-type channels in Purkinje neuron physiology has yet to be fully defined. In the current study we used physiological recordings from cultured Purkinje neurons and the L-type Ca2+ channel agonist S-(-)-Bay K to assess a potential role for L-type Ca2+ channels in spike firing. Results show that Bay K alters current-evoked spike firing in young, immature Purkinje neurons without dendritic structure and in older, more mature Purkinje neurons with dendritic structure. Bay K also enhanced Ca2+ signals associated with the current-evoked spike firing. The effect of Bay K was more prominent in the young Purkinje neurons than in the older Purkinje neurons, suggesting that L-type Ca2+ channels may be more important in the Purkinje neuron physiology during the early stages of development rather than at mature stages. In the older Purkinje neurons, immunohistochemical studies using antibodies to L-type Ca2+ channels showed more intense immunolabeling in the somatic region than in the dendritic region. This result suggests that L-type Ca2+ channels may play a more important role in somatic physiology than dendritic physiology, whereas P-type channels may play a more important role in dendritic physiology.

subject areas

  • Action Potentials
  • Animals
  • Animals, Newborn
  • Calcium
  • Calcium Channel Agonists
  • Calcium Channels, L-Type
  • Calcium Signaling
  • Cell Differentiation
  • Cells, Cultured
  • Cerebellar Cortex
  • Dendrites
  • Immunohistochemistry
  • Patch-Clamp Techniques
  • Purkinje Cells
  • Rats
  • Rats, Sprague-Dawley
  • Synaptic Membranes
  • Synaptic Transmission
scroll to property group menus

Research

keywords

  • Bay K
  • culture
  • development
  • immunohistochemistry
  • neuronal physiology
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 1473-4222

Digital Object Identifier (DOI)

  • 10.1080/14734220600719692

PubMed ID

  • 16818389
scroll to property group menus

Additional Document Info

start page

  • 146

end page

  • 154

volume

  • 5

issue

  • 2

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support