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Auto-reactive B cells against peripheral antigen, desmoglein 3, escape from tolerance mechanism

Academic Article
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Overview

authors

  • Ota, Takayuki
  • Aoki-Ota, M.
  • Tsunoda, K.
  • Simoda, K.
  • Nishikawa, T.
  • Amagai, M.
  • Koyasu, S.

publication date

  • October 2004

journal

  • International Immunology  Journal

abstract

  • To examine the mechanism of B cell tolerance against natural peripheral self-antigen, we generated transgenic mice expressing IgM specific for desmoglein 3 (Dsg3) from AK7 monoclonal antibody which itself does not induce blisters. Dsg3 is mainly expressed on stratified squamous epithelium and is the target antigen of an autoimmune bullous disease, pemphigus vulgaris. Transgenic B cells reactive to Dsg3 were observed in the spleen and lymph node. Although these B cells are autoreactive, they did not develop into B1 B cells. These B cells were functionally competent and anti-Dsg3 IgM was detected in the serum and on the keratinocyte cell surface. These results indicate that auto-reactive B cells against peripheral antigen (Dsg3) are able to develop in the presence of Dsg3 but are ignored by the immune system.

subject areas

  • Adoptive Transfer
  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Autoantigens
  • Autoimmunity
  • B-Lymphocytes
  • Cytoskeletal Proteins
  • Desmoglein 3
  • Desmogleins
  • Desmoplakins
  • Enzyme-Linked Immunosorbent Assay
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Humans
  • Immune Tolerance
  • Immunoglobulin M
  • Immunoglobulin Variable Region
  • Lymph Nodes
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Recombinant Proteins
  • Spleen
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Research

keywords

  • B1B cell
  • autoimmunity
  • pemphigus vulgaris
  • peripheral tolerance
  • transgenic mouse
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Identity

International Standard Serial Number (ISSN)

  • 0953-8178

Digital Object Identifier (DOI)

  • 10.1093/intimm/dxh150

PubMed ID

  • 15339881
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Additional Document Info

start page

  • 1487

end page

  • 1495

volume

  • 16

issue

  • 10

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