Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

A brief period of hypoxia causes proteolysis of cytoskeletal proteins in hippocampal slices

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Arai, A.
  • Vanderklish, Peter
  • Kessler, M.
  • Lee, K.
  • Lynch, G.

publication date

  • August 1991

journal

  • Brain Research  Journal

abstract

  • Breakdown products (BDPs) resulting from the partial proteolysis of spectrin were examined in hippocampal slices after periods of hypoxia lasting for 5 or 10 min. The concentration of a approximately 155 kDa BDP increased nearly twofold after 5 min of hypoxia; further increases were not seen with 10 min episodes or 10 min of hypoxia followed by reoxygenation. The hypoxia-induced proteolysis was blocked by prior infusion of a newly introduced inhibitor of calpain (calpain inhibitor I, 200 microM). Together with previously published data showing improved recovery of hippocampal slices from hypoxia in the presence of calpain inhibitors, these data suggest that activation of calpain may contribute significantly to the pathophysiology of ischemia.

subject areas

  • Animals
  • Blotting, Western
  • Calpain
  • Cytoskeletal Proteins
  • Electrophoresis, Polyacrylamide Gel
  • Glycoproteins
  • Hippocampus
  • Hydrolysis
  • Hypoxia, Brain
  • In Vitro Techniques
  • Male
  • Rats
  • Rats, Inbred Strains
  • Spectrin
scroll to property group menus

Research

keywords

  • CALPAIN
  • CALPAIN INHIBITOR-I
  • HIPPOCAMPUS
  • ISCHEMIA
  • PROTEASE
  • SPECTRIN
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0006-8993

Digital Object Identifier (DOI)

  • 10.1016/0006-8993(91)90352-v

PubMed ID

  • 1933340
scroll to property group menus

Additional Document Info

start page

  • 276

end page

  • 280

volume

  • 555

issue

  • 2

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support