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Exploring the basis of peptide-carbohydrate crossreactivity: Evidence for discrimination by peptides between closely related anti-carbohydrate antibodies

Academic Article
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Overview

authors

  • Harris, S. L.
  • Craig, L.
  • Mehroke, J. S.
  • Rashed, M.
  • Zwick, Michael
  • Kenar, K.
  • Toone, E. J.
  • Greenspan, N.
  • Auzanneau, F. I.
  • MarinoAlbernas, J. R.
  • Pinto, B. M.
  • Scott, J. K.

publication date

  • 1997

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • To investigate the molecular basis of antigenic mimicry by peptides, we studied a panel of closely related mAbs directed against the cell-wall polysaccharide of group A Streptococcus. These antibodies have restricted V-gene usage, indicating a shared mechanism of binding to a single epitope. Epitope mapping studies using synthetic fragments of the cell-wall polysaccharide supported this conclusion. All of the mAbs isolated crossreactive peptides from a panel of phage-displayed libraries, and competition studies indicated that many of the peptides bind at or near the carbohydrate binding site. Surprisingly, the peptides isolated by each mAb fell into distinct consensus-sequence groups that discriminated between the mAbs, and in general, the peptides bound only to the mAbs used for their isolation. Similar results were obtained with polyclonal antibodies directed against synthetic oligosaccharide fragments of the streptococcal cell-wall polysaccharide. Thus, the peptides appear to be specific for their isolating antibodies and are not recognized by the same mechanism as their carbohydrate counterparts.

subject areas

  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Antibody Specificity
  • Carbohydrate Sequence
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Immunoglobulin G
  • Immunoglobulin M
  • Immunoglobulin kappa-Chains
  • Molecular Sequence Data
  • Oligopeptides
  • Oligosaccharides
  • Peptides
  • Polysaccharides, Bacterial
  • Salmonella
  • Shigella flexneri
  • Streptococcus pyogenes
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Research

keywords

  • carbohydrate mimicry
  • group A Streptococcus
  • phage-displayed peptide libraries
  • synthetic oligosaccharide mapping
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.94.6.2454

PubMed ID

  • 9122216
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Additional Document Info

start page

  • 2454

end page

  • 2459

volume

  • 94

issue

  • 6

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