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Formation of an rna heterodimer upon heating of nodavirus particles

Academic Article
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Overview

authors

  • Krishna, N. K.
  • Schneemann, Anette

publication date

  • February 1999

journal

  • Journal of Virology  Journal

abstract

  • Flock House virus is a small icosahedral insect virus of the family Nodaviridae. Its genome consists of two positive-sense RNA molecules, which are believed to be encapsidated into a single viral particle. However, evidence to support this claim is circumstantial. Here we demonstrate that exposure of nodavirus particles to heat causes the two strands of viral RNA to form a stable complex, directly establishing that both RNAs are copackaged into one virion. The physical properties of the RNA complex, the effect of heat on the particles per se, and the possible relevance of these findings to the nodavirus life cycle are presented.

subject areas

  • Animals
  • Dimerization
  • Heating
  • Insect Viruses
  • Insecta
  • RNA Viruses
  • RNA, Viral
  • Solutions
  • Virion
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Identity

PubMed Central ID

  • PMC104002

International Standard Serial Number (ISSN)

  • 0022-538X

PubMed ID

  • 9882383
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Additional Document Info

start page

  • 1699

end page

  • 1703

volume

  • 73

issue

  • 2

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