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Flavonoids protect retinal ganglion cells from ischemia in vitro

Academic Article
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Overview

authors

  • Maher, P.
  • Hanneken, Anne

publication date

  • February 2008

journal

  • Experimental Eye Research  Journal

abstract

  • Retinal ischemia is a common cause of visual impairment and blindness. However, despite the significant advances that have been made in understanding the pathophysiology of retinal ischemia, effective treatments are still lacking. The goal of these studies was to use an in vitro model to identify molecules that could be neuroprotective for retinal ganglion cells exposed to ischemia. Ischemia was induced in the rat retinal ganglion cell line, RGC-5, using iodoacetic acid (IAA). Brief treatment with IAA resulted in RGC-5 cell death within 24 h by a non-apoptotic mechanism. Similar to ischemia in vivo, IAA treatment caused a rapid loss of ATP to approximately 50% of control levels. In contrast, changes in markers of oxidative stress occurred more slowly and included an increase in reactive oxygen species and a decrease in glutathione. Specific flavonoids were able to prevent the cell death caused by IAA treatment. Some of the flavonoids also prevented the loss of ATP as well as the changes in markers of oxidative stress. In contrast, classical antioxidants had only a very modest effect on IAA-induced cell death. These results suggest that specific flavonoids may be useful in preventing ischemia-induced retinal ganglion cell death in vivo.

subject areas

  • Adenosine Triphosphate
  • Animals
  • Antioxidants
  • Cell Death
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Flavonoids
  • Glutathione
  • Iodoacetic Acid
  • Ischemia
  • Oxidative Stress
  • Rats
  • Reactive Oxygen Species
  • Retinal Ganglion Cells
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Research

keywords

  • ATP
  • glutathione
  • glycolysis
  • oxidative stress
  • reactive oxygen species
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Identity

International Standard Serial Number (ISSN)

  • 0014-4835

Digital Object Identifier (DOI)

  • 10.1016/j.exer.2007.11.009

PubMed ID

  • 18160067
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Additional Document Info

start page

  • 366

end page

  • 374

volume

  • 86

issue

  • 2

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