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Tissue injury caused by deposition of immune complexes is l-arginine dependent

Academic Article
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Overview

authors

  • Mulligan, M. S.
  • Hevel, J. M.
  • Marletta, Michael
  • Ward, P. A.

publication date

  • 1991

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Nitric oxide (NO.), a free radical that is generated from L-arginine by stimulated endothelial cells, neutrophils, activated macrophages, and other cell types, reacts with superoxide anion (O2.-) to form peroxynitrite, which itself may be tissue toxic or can then react further to form the highly reactive and toxic hydroxyl radical (HO.). Because vascular injury produced by tissue deposition of immune complexes is linked to formation of toxic products derived from activated neutrophils, we have assessed whether immune complex-induced injury of rat lung and dermal vasculature is arginine dependent. The arginine analogue, NG-monomethyl-L-arginine (N-MeArg), which blocks NO. formation, protects against immune complex-induced vascular injury in rats. The protective effects of N-MeArg are reversed by the presence of L-arginine but not D-arginine. Additionally, in the absence of N-MeArg, injury is enhanced by the presence of L-arginine but not by D-arginine. Protection by N-MeArg is not associated with diminished recruitment of polymorphonuclear leukocytes. Bronchoalveolar lavage fluids from animals undergoing immune complex deposition in lung contain the decomposition products of NO.--namely, nitrite and nitrate. In the presence of N-MeArg these products are greatly diminished. These data suggest that immune complex-induced injury of rat lung and skin is L-arginine dependent. These data also suggest that in vivo metabolic products of L-arginine, such as NO(.), are directly or indirectly linked to immune complex-induced tissue injury.

subject areas

  • Animals
  • Antigen-Antibody Complex
  • Arginine
  • Free Radicals
  • Inflammation
  • Lung
  • Male
  • Neutrophils
  • Nitric Oxide
  • Peroxidase
  • Rats
  • Skin
  • Superoxides
  • omega-N-Methylarginine
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Research

keywords

  • HYDROXYL RADICAL
  • NG-MONOMETHYL-L-ARGININE
  • NITRIC OXIDE
  • SUPEROXIDE
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Identity

PubMed Central ID

  • PMC52078

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.88.14.6338

PubMed ID

  • 1648737
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Additional Document Info

start page

  • 6338

end page

  • 6342

volume

  • 88

issue

  • 14

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