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Reduction of exogenous vasopressin rna poly(a) tail length increases its effectiveness in transiently correcting diabetes-insipidus in the brattleboro rat

Academic Article
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Overview

authors

  • Maciejewskilenoir, D.
  • Jirikowski, G. F.
  • Sanna, Pietro
  • Bloom, Floyd

publication date

  • February 1993

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Magnocellular hypothalamic neurons in Brattleboro rats can accumulate, transport, and translate exogenous [Arg8]vasopressin (AVP) mRNA after injection in the hypothalamo-hypophysial tract in amounts sufficient to reverse transiently the animals' characteristic diabetes insipidus. In the present study, different preparations of hypothalamic RNA extracted from normal rats or synthetic AVP RNA were injected into the lateral hypothalamus of Brattleboro rats. Poly(A)- RNA and poly(A)+ RNA from which tails were removed by RNase H digestion were much more effective than poly(A)+ RNA in expressing AVP in the magnocellular hypothalamic neurons and in raising urine osmolarity. Synthetic AVP RNA lacking a poly(A) tail also produced a very potent dose-dependent diabetes insipidus reversal. Our results suggest that a short or absent poly(A) tail may facilitate the accumulation, transport, or expression of exogenous AVP mRNA by magnocellular neurons.

subject areas

  • Animals
  • Arginine Vasopressin
  • Diabetes Insipidus
  • Dose-Response Relationship, Drug
  • Hypothalamo-Hypophyseal System
  • Hypothalamus
  • Poly A
  • RNA
  • RNA, Messenger
  • Rats
  • Rats, Brattleboro
  • Rats, Inbred Lew
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Research

keywords

  • ACCUMULATION AND AXONAL TRANSPORT OF RNA
  • INTRAHYPOTHALAMIC INJECTIONS OF RNA
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.90.4.1435

PubMed ID

  • 7679506
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Additional Document Info

start page

  • 1435

end page

  • 1439

volume

  • 90

issue

  • 4

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