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Cell interactions in the primary immune response in vitro: A requirement for specific cell clusters

Academic Article
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Overview

authors

  • Mosier, Donald

publication date

  • 1969

journal

  • Journal of Experimental Medicine  Journal

abstract

  • Mouse spleen cells were found to associate in cell clusters during the primary immune response to sheep erythrocytes in vitro. About 10% of the cell clusters had the following unique properties; (a) they contained most, if not all, antibody-forming cells, (b) they contained only cells forming antibody to one antigen when cell cultures were immunized with two antigens, (c) the cells in clusters reaggregated specifically after dispersion, and (d) the specific reaggregation of clusters appeared to be blocked by antibody to the antigen. The integrity of cell clusters was required for the proliferation of antibody-forming cells, and prevention of clustering by mechanical means or by excess antibody blocked the immune response. Antibody and antigenic determinants on the surfaces of cells probably provide the basis for interaction. The unique microenvironment of cell clusters was essential for the primary immune response in vitro.

subject areas

  • Animals
  • Antibody Formation
  • Antigen-Antibody Reactions
  • Carbon Isotopes
  • Cell Aggregation
  • Cell Membrane
  • Culture Techniques
  • Erythrocytes
  • Lymphocytes
  • Mice
  • Mitosis
  • Sheep
  • Spleen
  • Surface Properties
  • Thymidine
  • Time Factors
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Identity

PubMed Central ID

  • PMC2138600

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.129.2.351

PubMed ID

  • 5762048
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Additional Document Info

start page

  • 351

end page

  • 362

volume

  • 129

issue

  • 2

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