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Cytosolic prion protein in neurons

Academic Article
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Overview

authors

  • Mironov, A.
  • Latawiec, D.
  • Wille, H.
  • Bouzamondo-Bernstein, E.
  • Legname, G.
  • Williamson, R. A.
  • Burton, Dennis
  • DeArmond, S. J.
  • Prusiner, S. B.
  • Peters, P. J.

publication date

  • August 2003

journal

  • Journal of Neuroscience  Journal

abstract

  • Localizing the cellular prion protein (PrPC) in the brain is necessary for understanding the pathogenesis of prion diseases. However, the precise ultrastructural localization of PrPC still remains enigmatic. We performed the first quantitative study of the ultrastructural localization of PrPC in the mouse hippocampus using high-resolution cryoimmunogold electron microscopy. PrPC follows the standard biosynthetic trafficking pathway with a preferential localization in late endosomal compartments and on the plasma membrane of neurons and neuronal processes. PrPC is found with the same frequency within the synaptic specialization and perisynaptically, but is almost completely excluded from synaptic vesicles. Unexpectedly, PrP is also found in the cytosol in subpopulations of neurons in the hippocampus, neocortex, and thalamus but not the cerebellum. Cytosolic PrP may have altered susceptibility to aggregation, suggesting that these neurons might play a significant role in the pathogenesis of prion diseases, in particular those mammals harboring mutant PrP genes.

subject areas

  • Animals
  • Antibody Specificity
  • Cell Membrane
  • Cricetinae
  • Cytosol
  • Dendrites
  • Endosomes
  • Fluorescent Antibody Technique
  • Hippocampus
  • Immunohistochemistry
  • Mice
  • Mice, Inbred Strains
  • Mice, Transgenic
  • Neurons
  • Neuropil
  • Organelles
  • PrPC Proteins
  • Synaptic Vesicles
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Research

keywords

  • cytosolic
  • hippocampus
  • immunogold
  • localization
  • membrane
  • prion protein
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Identity

International Standard Serial Number (ISSN)

  • 0270-6474

PubMed ID

  • 12904479
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Additional Document Info

start page

  • 7183

end page

  • 7193

volume

  • 23

issue

  • 18

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