Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Ultrastructural-localization of viral-antigens in the cns of mice persistently infected with lymphocytic choriomeningitis virus (lcmv)

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Rodriguez, M.
  • Buchmeier, M. J.
  • Oldstone, Michael
  • Lampert, P. W.

publication date

  • 1983

journal

  • American Journal of Pathology  Journal

abstract

  • Lymphocytic choriomeningitis virus (LCMV) produces a persistent infection of the nervous system in susceptible mice. To map the localization of viral antigens in the central nervous system (CNS), the authors studied, by means of ultrastructural immune peroxidase techniques, 4-6-month-old mice persistently infected with LCMV following an intracerebral inoculation at birth. The greatest number of infected neurons was observed in the cortex, particularly of the limbic system, and certain nuclei of the hypothalamus. In the cerebellum, Purkinje cells selectively expressed viral antigens. Moderate numbers of infected neurons were found in the anterior horns of the spinal cord, basal ganglia, and thalamus. The immunoperoxidase technique using monoclonal antibodies showed that persistently infected neurons primarily expressed the nucleocapsid protein antigens of LCMV. Glycopeptide antigens were minimally expressed. Electron-microscopic examination of selected individual infected neurons showed viral antigens exclusively associated with ribosomes. No staining was seen on cell surfaces. Glutaraldehyde-fixed CNS tissue studied by electron microscopy did not reveal morphologic abnormalities or mature viral particles. This study demonstrates that LCMV persistently infects specific neuronal populations. Infected neurons express viral antigens in association with host ribosomes and show no significant morphologic alterations.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Viral
  • Brain
  • Cerebellum
  • Cerebral Cortex
  • Hippocampus
  • Immunoenzyme Techniques
  • Limbic System
  • Lymphocytic Choriomeningitis
  • Lymphocytic choriomeningitis virus
  • Mice
  • Microscopy, Electron
  • Neurons
scroll to property group menus

Identity

PubMed Central ID

  • PMC1916133

International Standard Serial Number (ISSN)

  • 0002-9440

PubMed ID

  • 6336906
scroll to property group menus

Additional Document Info

start page

  • 95

end page

  • 100

volume

  • 110

issue

  • 1

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support