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Inhibition by colchicine of mitogenic stimulation of lymphocytes prior to s-phase

Academic Article
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Overview

authors

  • Wang, J. L.
  • Gunther, G. R.
  • Edelman, Gerald

publication date

  • 1975

journal

  • Journal of Cell Biology  Journal

abstract

  • Colchicine, vinblastine, and vincristine inhibit the mitogenic stimulation of lymphocytes by concanavalin A as measured by the incorporation of [3H]thymidine and the appearance of blast cells. The inhibitory effect of colchicine could not be accounted for by diminution in cell viability or by metaphase arrest of mitosis in the stimulated cells. Moreover, the inhibition of [3H]thymidine incorporation was not due to blockage of thymidine transport or inhibition of DNA synthesis inasmuch as addition of colchicine had no effect on cells in the S phase of the cell cycle. The time of inhibition was correlated with the kinetics of cellular commitment to lectin activation and the kinetic data indicated that colchicine blocks stimulation early in the sequence of events following addition of the mitogen. These findings support the hypothesis that cytoplasmic microtubular function plays a role in the commitment of resting cells to undergo mitotic division.

subject areas

  • Animals
  • Autoradiography
  • Cells, Cultured
  • Colchicine
  • Concanavalin A
  • DNA
  • Drug Antagonism
  • Humans
  • Kinetics
  • Lymphocyte Activation
  • Lymphocytes
  • Methylmannosides
  • Mice
  • Rabbits
  • Thymidine
  • Tritium
  • Vinblastine
  • Vincristine
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Identity

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.66.1.128

PubMed ID

  • 1170172
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Additional Document Info

start page

  • 128

end page

  • 144

volume

  • 66

issue

  • 1

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