Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Characterization of murine a-raf, a new oncogene related to the v-raf oncogene

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Huleihel, M.
  • Goldsborough, M.
  • Cleveland, John
  • Gunnell, M.
  • Bonner, T.
  • Rapp, U. R.

publication date

  • 1986

journal

  • Molecular and Cellular Biology  Journal

abstract

  • A 1.6-kilobase cDNA (A-raf) has been isolated from a murine spleen cDNA library which encodes part of a protein related to the raf oncogene. Its amino acid sequence has 85% homology to raf in a central portion of 100 amino acids. In contrast to raf, A-raf shows a highly restricted tissue distribution of expression, with highest levels observed in epididymis, followed by intestine. When incorporated into a retrovirus, the resulting gag-A-raf fusion gene causes transformation in vitro and induces tumors in newborn mice. Thus, A-raf represents a new proto-oncogene. Transformation by A-raf is independent of ras gene function, as is the case for raf and mos but not other oncogenes.

subject areas

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • DNA
  • Gene Expression Regulation
  • Mice
  • Oncogenes
  • Spleen
  • Tissue Distribution
  • Viral Proteins
scroll to property group menus

Identity

PubMed Central ID

  • PMC367822

International Standard Serial Number (ISSN)

  • 0270-7306

PubMed ID

  • 3491291
scroll to property group menus

Additional Document Info

start page

  • 2655

end page

  • 2662

volume

  • 6

issue

  • 7

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support