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Combinatorial alanine substitution enables rapid optimization of cytochrome p450(bm3) for selective hydroxylation of large substrates

Academic Article
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Overview

authors

  • Lewis, J. C.
  • Mantovani, S. M.
  • Fu, Y.
  • Snow, C. D.
  • Komor, R. S.
  • Wong, Chi-Huey
  • Arnold, F. H.

publication date

  • December 2010

journal

  • ChemBioChem  Journal

subject areas

  • Alanine
  • Alkaloids
  • Bacillus megaterium
  • Bacterial Proteins
  • Cytochrome P-450 Enzyme System
  • Hydroxylation
  • Monosaccharides
  • NADPH-Ferrihemoprotein Reductase
  • Steroids
  • Substrate Specificity
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Research

keywords

  • alkaloids
  • biocatalysis
  • cytochromes
  • monosaccharides
  • steroids
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Identity

PubMed Central ID

  • PMC4447097

International Standard Serial Number (ISSN)

  • 1439-4227

Digital Object Identifier (DOI)

  • 10.1002/cbic.201000565

PubMed ID

  • 21108271
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Additional Document Info

start page

  • 2502

end page

  • 2505

volume

  • 11

issue

  • 18

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