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Mechanisms of antibody-binding to a protein

Academic Article
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Overview

authors

  • Getzoff, Elizabeth
  • Geysen, H. M.
  • Rodda, S. J.
  • Alexander, H.
  • Tainer, John
  • Lerner, Richard

publication date

  • March 1987

journal

  • Science  Journal

abstract

  • The mechanisms of antibody binding to a protein were studied by an analysis of specific amino acid residues critical to nine antigenic sites on myohemerythrin. Rabbit antisera to the whole protein were assayed for binding to more than 1500 distinct peptide analogs differing from the protein sequence by single amino acid replacements. The results, combined with information from the three-dimensional crystallographic structure, were used to evaluate probable mechanisms of antibody binding at individual sites. The data from all sites examined indicate that initial binding to solvent-exposed amino acid residues may promote local side-chain displacements and thereby allow the participation of other, previously buried, residues.

subject areas

  • Animals
  • Antibodies
  • Binding, Competitive
  • Biomechanical Phenomena
  • Computer Graphics
  • Hemerythrin
  • Metalloproteins
  • Molecular Conformation
  • Rabbits
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Identity

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.3823879

PubMed ID

  • 3823879
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Additional Document Info

start page

  • 1191

end page

  • 1196

volume

  • 235

issue

  • 4793

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