Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Integrin alpha-v-beta-5 selectively promotes adenovirus-mediated cell-membrane permeabilization

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Wickham, T. J.
  • Filardo, E. J.
  • Cheresh, D. A.
  • Nemerow, Glen

publication date

  • October 1994

journal

  • Journal of Cell Biology  Journal

abstract

  • Human adenovirus type 2 (Ad2) enters host cells by receptor-mediated endocytosis, an event mediated by the virus penton base binding to cell surface integrins alpha v beta 3 and alpha v beta 5. While both alpha v integrins promote virus internalization, alpha v beta 5 is involved in the subsequent event of membrane permeabilization. Cells transfected with the beta 5 or beta 3 subunit, expressing either alpha v beta 5 and alpha v beta 3, respectively, were capable of supporting Ad2 infection to varying degrees. In this case, cells expressing alpha v beta 5 were significantly more susceptible to Ad2-induced membrane permeabilization, as well as to Ad2 infection, than cells expressing alpha v beta 3. Adenovirus-mediated gene delivery was also more efficient in cells expressing alpha v beta 5. These results suggest that the interaction of alpha v beta 5 with Ad2 penton base facilitates the subsequent step of virus penetration into the cell. These studies provide evidence for the involvement of a cellular receptor in virus-mediated membrane permeabilization and suggest a novel biological role for integrin alpha v beta 5 in the infectious pathway of a human adenovirus.

subject areas

  • Adenoviruses, Human
  • Azides
  • Capsid
  • Capsid Proteins
  • Cell Adhesion
  • Cell Line
  • Cell Membrane Permeability
  • Endocytosis
  • Gene Expression
  • Glucuronidase
  • Humans
  • Integrins
  • Receptors, Cytoadhesin
  • Receptors, Virus
  • Receptors, Vitronectin
  • Recombinant Proteins
  • Sodium Azide
  • Transfection
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.127.1.257

PubMed ID

  • 7523420
scroll to property group menus

Additional Document Info

start page

  • 257

end page

  • 264

volume

  • 127

issue

  • 1

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support