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Slx1-Slx4 are subunits of a structure-specific endonuclease that maintains ribosomal DNA in fission yeast

Academic Article
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Overview

authors

  • Coulon, S.
  • Gaillard, P. H. L.
  • Chahwan, C.
  • McDonald, W. H.
  • Yates III, John
  • Russell, Paul

publication date

  • 2004

journal

  • Molecular Biology of the Cell  Journal

abstract

  • In most eukaryotes, genes encoding ribosomal RNAs (rDNA) are clustered in long tandem head-to-tail repeats. Studies of Saccharomyces cerevisiae have indicated that rDNA copy number is maintained through recombination events associated with site-specific blockage of replication forks (RFs). Here, we describe two Schizosaccharomyces pombe proteins, homologs of S. cerevisiae Slx1 and Slx4, as subunits of a novel type of endonuclease that maintains rDNA copy number. The Slx1-Slx4-dependent endonuclease introduces single-strand cuts in duplex DNA on the 3' side of junctions with single-strand DNA. Deletion of Slx1 or Rqh1 RecQ-like DNA helicase provokes rDNA contraction, whereas simultaneous elimination of Slx1-Slx4 endonuclease and Rqh1 is lethal. Slx1 associates with chromatin at two foci characteristic of the two rDNA repeat loci in S. pombe. We propose a model in which the Slx1-Slx4 complex is involved in the control of the expansion and contraction of the rDNA loci by initiating recombination events at stalled RFs.

subject areas

  • Chromatin
  • DNA, Ribosomal
  • DNA-Binding Proteins
  • Endonucleases
  • Microscopy, Fluorescence
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Subunits
  • Recombination, Genetic
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Sequence Alignment
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Identity

PubMed Central ID

  • PMC307528

International Standard Serial Number (ISSN)

  • 1059-1524

Digital Object Identifier (DOI)

  • 10.1091/mbc.E03-08-0586

PubMed ID

  • 14528010
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Additional Document Info

start page

  • 71

end page

  • 80

volume

  • 15

issue

  • 1

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