Applications of laser-polarized (129)Xe to biomolecular assays

Academic Article

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• Research
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Overview

authors

• Lowery, T. J.
• Rubin, S. M.
• Ruiz, E. J.
• Spence, M. M.
• Winssinger, Nicolas
• Schultz, Peter
• Pines, Alexander
• Wemmer, D. E.

publication date

• 2003

journal

• Magnetic Resonance Imaging  Journal

abstract

• The chemical shift sensitivity and significant signal enhancement afforded by laser-polarized 129Xe have motivated the application of 129Xe NMR to biological imaging and spectroscopy. Recent research done by our group has used laser-polarized 129Xe in biomolecular assays that detect ligand-binding events and distinguish protein conformations. The successful application of unfunctionalized and functionalized 129Xe NMR to in vitro biomolecular assays suggests the potential future use of a functionalized xenon biosensor for in vivo imaging.

subject areas

• Humans
• Lasers
• Ligands
• Nuclear Magnetic Resonance, Biomolecular
• Protein Conformation
• Xenon Isotopes

Research

keywords

• biosensor
• ligand-protein interactions
• protein conformation assay
• xenon binding

Identity

International Standard Serial Number (ISSN)

• 0730-725X

Digital Object Identifier (DOI)

• 10.1016/j.mri.2003.08.025

PubMed ID

• 14725931

Additional Document Info

start page

• 1235

end page

• 1239

volume

• 21

issue

• 10