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Physical association of histocompatibility antigens and tumor-associated antigens on the surface of murine lymphoma-cells

Academic Article
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Overview

authors

  • Callahan, G. N.
  • Allison, J. P.
  • Pellegrino, M. A.
  • Reisfeld, Ralph

publication date

  • 1979

journal

  • Journal of Immunology  Journal

abstract

  • Serologic and immunochemical techniques were used to characterize plasma membrane antigens of the murine lymphoma 6C3HED. Syngeneic C3H/HeJ anti-6C3HED antisera were prepared and were shown by cytotoxicity, absorption, and immunoprecipitation tests to be specifically reactive with the lymphoma cells. Alloantisera to H-2.23 were found to be unreactive with intact lymphoma cells by cytotoxicity and absorption assays. However, H-2.23 antigens were detectable in tumor cell lysates by inhibition of cytotoxicity and immunoprecipitation. SDS-PAGE electropherograms of immunoprecipitates of radiolabeled tumor cell extracts obtained with anti-6C3HED and anti-H2.23 antisera were essentially identical; proteins with Mr of approximately 70,000, 45,000, and 12,000 were apparent in both. An immuno-adsorbent of insolubilized anti-6C3HED removed H-2.23 antigens detectable in lymphoma extracts, but had no effect on H-2.23 antigens in normal splenocyte extracts. These data indicate that H-2.23 and TAA are physically associated on 6C3HED cells.

subject areas

  • Animals
  • Antibody Specificity
  • Antigens, Neoplasm
  • Antigens, Surface
  • Electrophoresis, Polyacrylamide Gel
  • H-2 Antigens
  • Immune Sera
  • Immunosorbents
  • Lymphoma
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 762424
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Additional Document Info

start page

  • 70

end page

  • 74

volume

  • 122

issue

  • 1

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