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Opening the high-pressure domain beyond 2 kbar to protein and virus crystallography - technical advance

Academic Article
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Overview

authors

  • Fourme, R.
  • Ascone, I.
  • Kahn, R.
  • Mezouar, M.
  • Bouvier, P.
  • Girard, E.
  • Lin, T. W.
  • Johnson Jr., John

publication date

  • 2002

journal

  • Structure  Journal

abstract

  • The combined use of a diamond anvil cell and ultrashort-wavelength undulator radiation has allowed the collection of high-resolution diffraction data from protein and virus crystals submitted to hydrostatic pressures beyond 2 kbar. Crystals of cubic cowpea mosaic virus (CPMV) can be compressed to at least 3.5 kbar. Diffraction from CPMV crystals displaying an unusual disorder at atmospheric pressure was considerably enhanced by application of pressure. These experiments suggest that pressure may be used in some cases to improve order in crystals.

subject areas

  • Crystallography
  • Pressure
  • Proteins
  • Viruses
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Research

keywords

  • crystals
  • high pressure
  • macromolecular crystallography
  • order
  • proteins
  • virus
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Identity

International Standard Serial Number (ISSN)

  • 0969-2126

Digital Object Identifier (DOI)

  • 10.1016/s0969-2126(02)00850-x

PubMed ID

  • 12377126
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Additional Document Info

start page

  • 1409

end page

  • 1414

volume

  • 10

issue

  • 10

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