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Crystallization of murine major histocompatibility complex class-i h-2kb with single peptides

Academic Article
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Overview

authors

  • Stura, E. A.
  • Matsumura, M.
  • Fremont, D. H.
  • Saito, Y.
  • Peterson, P. A.
  • Wilson, Ian

publication date

  • December 1992

journal

  • Journal of Molecular Biology  Journal

abstract

  • X-ray quality crystals of a soluble murine class I H-2Kb molecule complexed with three different peptide antigens were grown in several forms by streak seeding and macroseeding methods. Co-crystals with VSV-8 (RGYVYGQL), OVA-8 (SIINFEKL) and SEV-9 (FAPGNYPAL) peptides were grown either from NaH2PO4/HPO4 or from polyethylene glycol 4000 within the pH range 5.0 to 7.5, with the use of 4-methyl-2-pentane diol (MPD) as an additive. The VSV-8 crystals grew in space groups P1, with cell dimensions a = 63.1 A, b = 69.1 A, c = 72.0 A, alpha = 89.9 degrees, beta = 77.1 degrees, gamma = 123.3 degrees and P2(1)2(1)2, with a = 138.1 A, b = 88.6 A, c = 45.7 A, and diffract to 2.9 and 2.3 A, respectively. Crystals of the SEV-9 complex grew from similar crystallization conditions to those of the orthorhombic VSV-8 complex with similar cell parameters and diffract to at least 2.5 A resolution. Crystals of the OVA-8 complex were obtained from either phosphate (space group C2, a = 118.7 A, b = 61.6 A, c = 85.3 A, beta = 108.4 degrees) or polyethylene glycol (space group P1, a = 64.5 A, b = 71.0 A, c = 66.3 A, alpha = 89.7 degrees, beta = 95.7 degrees, gamma = 123.3 degrees) and diffract to 3 A resolution. The crystallization procedures used here significantly increased the rate and production of X-ray quality crystals.

subject areas

  • Amino Acid Sequence
  • Animals
  • Crystallization
  • Genes, MHC Class I
  • H-2 Antigens
  • Ligands
  • Macromolecular Substances
  • Major Histocompatibility Complex
  • Mice
  • Molecular Sequence Data
  • Peptides
  • X-Ray Diffraction
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Research

keywords

  • ADDITIVES
  • H-2KB
  • MHC CLASS-I
  • PEPTIDE COMPLEX
  • PROTEIN CRYSTALLIZATION
  • X-RAY CRYSTALLOGRAPHY
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Identity

International Standard Serial Number (ISSN)

  • 0022-2836

Digital Object Identifier (DOI)

  • 10.1016/0022-2836(92)90881-j

PubMed ID

  • 1469730
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Additional Document Info

start page

  • 975

end page

  • 982

volume

  • 228

issue

  • 3

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