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RCADiA: simple automation platform for comparative multidimensional protein identification technology

Academic Article
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Overview

authors

  • Bailey, A. O.
  • Miller, T. M.
  • Dong, M. Q.
  • Velde, C. V.
  • Cleveland, D. W.
  • Yates III, John

publication date

  • August 2007

journal

  • Analytical Chemistry  Journal

abstract

  • Multidimensional liquid chromatography in combination with tandem mass spectrometry has been used to analyze a variety of biological structures including protein complexes. Incorporating this approach with autosampling devices presents a number of problems including decreased sensitivity due to exposure to extra surfaces, carryover from run to run, and increased dead volume. We developed a device, termed Radial Column Array for Distribution and Automation (RCADiA), to automate multiple MuDPIT experiments while eliminating many of these problems and maintaining a high resolution and sensitive analysis. The design, which places each sample downstream of any common fluid path, presents a low risk of carryover between successive analyses. Beyond the convenience of automation, the RCADiA platform also produces data of similar quality to the standard method of performing individual MuDPIT experiments. We demonstrate this device by performing a comparative analysis of mitochondria enriched from rat liver and spinal cord.

subject areas

  • Animals
  • Automation
  • Chromatography, Liquid
  • Equipment Design
  • Liver
  • Mitochondria
  • Multiprotein Complexes
  • Proteins
  • Rats
  • Spinal Cord
  • Tandem Mass Spectrometry
  • Technology
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Identity

PubMed Central ID

  • PMC2528021

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac070585g

PubMed ID

  • 17616168
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Additional Document Info

start page

  • 6410

end page

  • 6418

volume

  • 79

issue

  • 16

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