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Targeting cytotoxic t-cells to antigen-specific lymphocytes-b

Academic Article
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Overview

authors

  • Rammensee, H. G.
  • Julius, M. H.
  • Nemazee, David
  • Langhorne, J.
  • Lamers, R.
  • Kohler, G.

publication date

  • March 1987

journal

  • European Journal of Immunology  Journal

abstract

  • A recent development in immunomanipulation involves the targeting of cytotoxic T lymphocytes (CTL) to cell-bound antigens using bispecific antibodies. These antibodies have been engineered such that specificity is directed against the T cell receptor (TCR) or TCR-associated T3 molecules, as well as against the chosen antigen. The present study was aimed to force interactions between T and B cells by bridging their receptors. F23.1 antibodies, which are specific for gene products of the TCR V beta 8 gene family, were conjugated with TNP (2,4,6-trinitrophenyl) and this construct was used to bridge the receptors of V beta 8+ T cells with the receptors of TNP-specific B cells. The bridging was demonstrated by direct killing of both a TNP-specific B hybridoma and of blast cells from mice transgenic for mu, kappa of the TNP-specific antibody Sp6. Further, F23.1-TNP constructs in conjunction with V beta 8+ CTL were shown to specifically deplete Ig-secreting B cells from Sp6 transgenic mice. Conjugates of TCR-specific antibodies and antigen are theoretically useful in vivo to either deplete or expand B cells of a given specificity by coupling their receptors to the TCR of CTL or T helper cells, respectively.

subject areas

  • Animals
  • Antibody Formation
  • B-Lymphocytes
  • Cytotoxicity, Immunologic
  • Immunity, Cellular
  • Immunoglobulin Fab Fragments
  • Mice
  • Receptors, Antigen, B-Cell
  • Receptors, Antigen, T-Cell
  • T-Lymphocytes, Cytotoxic
  • Trinitrobenzenes
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Identity

International Standard Serial Number (ISSN)

  • 0014-2980

Digital Object Identifier (DOI)

  • 10.1002/eji.1830170322

PubMed ID

  • 3494614
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Additional Document Info

start page

  • 433

end page

  • 436

volume

  • 17

issue

  • 3

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