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Use of multiple anomalous dispersion to phase highly merohedrally twinned crystals of interleukin-1 beta

Academic Article
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Overview

related to degree

  • Kelker, Matthew, Ph.D. in Biophysics, Scripps Research 2000 - 2005

authors

  • Rudolph, M. G.
  • Kelker, Matthew
  • Schneider, T. R.
  • Yeates, Todd O.
  • Oseroff, V.
  • Heidary, D. K.
  • Jennings, P. A.
  • Wilson, Ian

publication date

  • February 2003

journal

  • Acta Crystallographica Section D-Biological Crystallography  Journal

abstract

  • The crystal structure at 1.54 A resolution of a double mutant of interleukin-1beta (F42W/W120F), a cytokine secreted by macrophages, was determined by multiple-wavelength anomalous dispersion (MAD) using data from highly twinned selenomethionine-modified crystals. The space group is P4(3), with unit-cell parameters a = b = 53.9, c = 77.4 A. Self-rotation function analysis and various intensity statistics revealed the presence of merohedral twinning in crystals of both the native (twinning fraction alpha approximately 0.35) and SeMet (alpha approximately 0.40) forms. Structure determination and refinement are discussed with emphasis on the possible reasons for successful phasing using untreated twinned MAD data.

subject areas

  • Crystallization
  • Crystallography, X-Ray
  • Interleukin-1
  • Macrophages
  • Models, Molecular
  • Protein Conformation
  • Software
  • Static Electricity
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Identity

International Standard Serial Number (ISSN)

  • 0907-4449

Digital Object Identifier (DOI)

  • 10.1107/s0907444902021704

PubMed ID

  • 12554939
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Additional Document Info

start page

  • 290

end page

  • 298

volume

  • 59

issue

  • Pt 2

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