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Structure and function of a genetically engineered mimic of a nonenveloped virus entry intermediate

Academic Article
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Overview

authors

  • Banerjee, M.
  • Speir, J. A.
  • Kwan, M. H.
  • Huang, R.
  • Aryanpur, P. P.
  • Bothner, B.
  • Johnson Jr., John

publication date

  • May 2010

journal

  • Journal of Virology  Journal

abstract

  • Divalent metal ions are components of numerous icosahedral virus capsids. Flock House virus (FHV), a small RNA virus of the family Nodaviridae, was utilized as an accessible model system with which to address the effects of metal ions on capsid structure and on the biology of virus-host interactions. Mutations at the calcium-binding sites affected FHV capsid stability and drastically reduced virus infectivity, without altering the overall architecture of the capsid. The mutations also altered the conformation of gamma, a membrane-disrupting, virus-encoded peptide usually sequestered inside the capsid, by increasing its exposure under neutral pH conditions. Our data demonstrate that calcium binding is essential for maintaining a pH-based control on gamma exposure and host membrane disruption, and they reveal a novel rationale for the metal ion requirement during virus entry and infectivity. In the light of the phenotypes displayed by a calcium site mutant of FHV, we suggest that this mutant corresponds to an early entry intermediate formed in the endosomal pathway.

subject areas

  • Animals
  • Binding Sites
  • Calcium
  • Capsid Proteins
  • Cations, Divalent
  • Cell Line
  • Crystallography, X-Ray
  • Drosophila melanogaster
  • Host-Pathogen Interactions
  • Models, Molecular
  • Mutagenesis, Site-Directed
  • Nodaviridae
  • Protein Structure, Quaternary
  • Spodoptera
  • Viral Plaque Assay
  • Virus Internalization
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Identity

PubMed Central ID

  • PMC2863772

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.02670-09

PubMed ID

  • 20164221
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Additional Document Info

start page

  • 4737

end page

  • 4746

volume

  • 84

issue

  • 9

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