Purification and crystallization of a schistosomal glutathione-s-transferase

Academic Article

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Overview

authors

• McTigue, M. A.
• Bernstein, S. L.
• Williams, D. R.
• Tainer, John

publication date

• May 1995

journal

• Proteins-Structure Function and Genetics  Journal

abstract

• The 26-kDa glutathione S-transferase from Schistosoma japonica (Sj26), a potential antischistosomal vaccine antigen, has been crystallized in an unligated form. Sj26 was recombinantly produced in E. coli without using a glutathione affinity column to facilitate preparation of unligated enzyme. The recombinant protein contains all 218 residues of Sj26 and an additional 13 residues linked to the C-terminus. Crystals of recombinant Sj26 were obtained by the vapor diffusion method using ammonium sulfate as the precipitant at pH 5.6. The crystals belong to the hexagonal space group P6(3)22 with unit cell dimensions a = b = 125.2 A and c = 72.0 A and contain one Sj26 monomer per asymmetric unit. A complete native diffraction data set has been obtained to 2.4 A resolution.

subject areas

• Animals
• Antigens, Helminth
• Crystallization
• Crystallography, X-Ray
• Escherichia coli
• Glutathione Transferase
• Recombinant Proteins
• Schistosoma

Research

keywords

• CRYSTALLIZATION
• GLUTATHIONE S-TRANSFERASE
• HELMINTH
• SCHISTOSOMIASIS
• X-RAY DIFFRACTION

Identity

International Standard Serial Number (ISSN)

• 0887-3585

Digital Object Identifier (DOI)

• 10.1002/prot.340220108

PubMed ID

• 7675787

Additional Document Info

start page

• 55

end page

• 57

volume

• 22

issue

• 1