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TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity in Saccharomyces cerevisiae

Academic Article
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Overview

authors

  • Reinke, A.
  • Anderson, S.
  • McCaffery, J. M.
  • Yates III, John
  • Aronova, S.
  • Chu, S.
  • Fairclough, S.
  • Iverson, C.
  • Wedaman, K. P.
  • Powers, T.

publication date

  • April 2004

journal

  • Journal of Biological Chemistry  Journal

abstract

  • The Tor1p and Tor2p kinases, targets of the therapeutically important antibiotic rapamycin, function as components of two distinct protein complexes in yeast, termed TOR complex 1 (TORC1) and TORC2. TORC1 is responsible for a wide range of rapamycin-sensitive cellular activities and contains, in addition to Tor1p or Tor2p, two highly conserved proteins, Lst8p and Kog1p. By identifying proteins that co-purify with Tor1p, Tor2p, Lst8p, and Kog1p, we have characterized a comprehensive set of protein-protein interactions that define further the composition of TORC1 as well as TORC2. In particular, we have identified Tco89p (YPL180w) and Bit61p (YJL058c) as novel components of TORC1 and TORC2, respectively. Deletion of TOR1 or TCO89 results in two specific and distinct phenotypes, (i) rapamycin-hypersensitivity and (ii) decreased cellular integrity, both of which correlate with the presence of SSD1-d, an allele of SSD1 previously associated with defects in cellular integrity. Furthermore, we link Ssd1p to Tap42p, a component of the TOR pathway that is believed to act uniquely downstream of TORC1. Together, these results define a novel connection between TORC1 and Ssd1p-mediated maintenance of cellular integrity.

subject areas

  • Adaptor Proteins, Signal Transducing
  • Alleles
  • Antibiotics, Antineoplastic
  • Electrophoresis, Polyacrylamide Gel
  • Fungal Proteins
  • Gene Deletion
  • Mass Spectrometry
  • Microscopy, Immunoelectron
  • Phenotype
  • Phosphatidylinositol 3-Kinases
  • Phosphotransferases (Alcohol Group Acceptor)
  • Plasmids
  • Precipitin Tests
  • Protein Binding
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sirolimus
  • Temperature
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.M313062200

PubMed ID

  • 14736892
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Additional Document Info

start page

  • 14752

end page

  • 14762

volume

  • 279

issue

  • 15

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