Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Tumor necrosis factor in mediating experimental hemophilus-influenzae type-b meningitis

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Mustafa, M. M.
  • Ramilo, O.
  • Olsen, K. D.
  • Franklin, P. S.
  • Hansen, E. J.
  • Beutler, Bruce
  • McCracken, G. H.

publication date

  • October 1989

journal

  • Journal of Clinical Investigation  Journal

abstract

  • Tumor necrosis factor (TNF) could possibly be instrumental in mediating injury to the CNS during bacterial meningitis. In CSF of rabbits with meningitis induced with Haemophilus influenzae type b (Hib) lipooligosaccharide (LOS), TNF activity was first detected 45 min after intracisternal (IC) injection of 20 ng Hib LOS and white blood cells (WBC) first appeared 75 min later. The peak TNF activity (45 ng/ml) was observed at 120 min after IC and persisted for 5 h. When 1-2 X 10(7) CFU of Hib was used to induce meningitis, peak CSF TNF activity was comparable with that after 20 ng Hib LOS, but the activity persisted for 14 h. Dexamethasone (DXM) (1 mg/kg per i.v.) given 1 h before or simultaneously with IC Hib LOS reduced significantly TNF activity and meningeal inflammation. Goat anti-human TNF alpha antibodies given IC with 20 ng Hib LOS or 2 X 10(6) CFU of Hib resulted in a significant reduction in CSF TNF concentrations, which was also associated with reduced meningeal inflammation in Hib LOS-inoculated animals. We conclude that TNF participates in mediating meningeal inflammation associated with Hib experimental meningitis, and that DXM, when given before or with Hib LOS, inhibits CSF TNF production and modulates the meningeal inflammatory response.

subject areas

  • Animals
  • Dexamethasone
  • Disease Models, Animal
  • Glucose
  • Haemophilus influenzae
  • Lactates
  • Lipopolysaccharides
  • Male
  • Meningitis, Haemophilus
  • Proteins
  • Rabbits
  • Tumor Necrosis Factor-alpha
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0021-9738

Digital Object Identifier (DOI)

  • 10.1172/jci114292

PubMed ID

  • 2794062
scroll to property group menus

Additional Document Info

start page

  • 1253

end page

  • 1259

volume

  • 84

issue

  • 4

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support