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High anti-TNP plaque-forming cell potential of residual mIg+ cells in a T cell population

Academic Article
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Overview

authors

  • Mond, J. J.
  • Mage, M. G.
  • Rothstein, T. L.
  • Mosier, Donald
  • Herrod, H.
  • Asofsky, R.
  • Paul, W. E.

publication date

  • 1980

journal

  • Journal of Immunology  Journal

abstract

  • In the course of experiments designed to study the immune response of purified populations of B lymphocytes to thymus-independent (TI) antigens, a variety of cell purification procedures were followed. In using anti-immunoglobulin-coated dishes to separate lymphocytes bearing membrane immunoglobulin (mIg) from mIg- lymphocytes, it was found that the nonadherent fraction, which was predominantly mIg-, complement receptor negative, and nonresponsive to the B cell mitogen lipopolysaccharide, gave very substantial anti-TNP plaque-forming cell responses to 2 TI antigens. These responses could be inhibited by incubation of such cells in the presence of anti-mu and thus appeared to be attributable to mIg+ cells. The evidence suggests the existence of a population of B lymphocytes that constitute a minor component of mIg+ cells having a high potential to make in vitro antibody responses. Users of techniques that utilize anti-Ig as a tool for separating B and T lymphocytes should carefully assess the extent to which residual B lymphocytes in the mIg- population contribute to antibody responses being studied.

subject areas

  • Animals
  • Antibody Formation
  • Antibody-Producing Cells
  • B-Lymphocytes
  • Brucella abortus
  • Cell Adhesion
  • Hemolytic Plaque Technique
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Inbred DBA
  • Nitrobenzenes
  • Phenotype
  • Receptors, Antigen, B-Cell
  • T-Lymphocytes
  • Trinitrobenzenes
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 6774022
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Additional Document Info

start page

  • 1526

end page

  • 1529

volume

  • 125

issue

  • 4

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