Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Immune-response to uncoupled peptides of foot-and-mouth-disease virus

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Francis, M. J.
  • Fry, C. M.
  • Rowlands, D. J.
  • Bittle, J. L.
  • Houghten, R. A.
  • Lerner, Richard
  • Brown, F.

publication date

  • May 1987

journal

  • Immunology  Journal

abstract

  • Uncoupled synthetic peptide representing the sequence of amino acids 141-160 of foot-and-mouth disease virus (FMDV) protein VP1 induced a virus-neutralizing antibody response in guinea-pigs. This response required incomplete Freund's adjuvant (IFA) for the primary inoculation and was dependent on the presence of an added cysteine residue with an unblocked sulphydryl group at the carboxy-terminus. Secondary immunization could be carried out in the absence of adjuvant. A study of the relative activities of nested sets of uncoupled peptides from 150-160 to 135-160 and 141-160 to 141-155 indicated that amino acids 146-156 were critical for the induction of virus-neutralizing antibodies and that extension to 137-160 further improved this response. Results of in vitro proliferation studies demonstrated that the carboxy-terminal residues on this peptide may form a T-cell epitope. The significance of these observations in the broader context of synthetic peptide vaccines is discussed.

subject areas

  • Adjuvants, Immunologic
  • Animals
  • Antibodies, Viral
  • Aphthovirus
  • Cysteine
  • Female
  • Guinea Pigs
  • Immunologic Memory
  • Lymphocyte Activation
  • Peptide Fragments
  • T-Lymphocytes
  • Viral Proteins
scroll to property group menus

Identity

PubMed Central ID

  • PMC1453305

International Standard Serial Number (ISSN)

  • 0019-2805

PubMed ID

  • 3034769
scroll to property group menus

Additional Document Info

start page

  • 1

end page

  • 6

volume

  • 61

issue

  • 1

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support